Featured Publications

Bissig-Choisat B, Wang L, Legras X, Pradip KS, Chen L, Bell P, Pankowicz FP, Hill MC, Barzi M, Kettlun Leyton C, Eastwood LC, Kruse RL, Himes RW, Goss JA, Wilson JM, Chan LL, Lagor WR and Bissig KD Development and rescue of human familial hypercholesterolemia in a xenograft mouse model. Nat Commun. 2015 June. 6:7339. PMID: 26081744

Dysregulation of lipid metabolism is associated with cardiovascular disease, metabolic syndrome, diabetes, and other conditions that have become significant health problems. Unfortunately, species differences in lipid metabolism have made it difficult to model lipid pathways in rodents or draw reliable conclusions from animal studies. Here we transplanted liver cells from human patients with familial hypercholesterolemia, a severe form of lipid dysregulation, and demonstrate not only that this mouse develops hypercholesterolemia that physiologically recapitulates the human disease, but also that gene therapy corrected the underlying defect and restored the human hepatocytes in the mouse to normal function. This was the first xenograft model for a metabolic liver disease that allowed the testing of a macromolecular therapy, which traditionally could be validated only in human clinical trials.

Pankowicz FP, Barzi M, Legras X, Hubert L, Mi T, Tomolonis JA, Ravishankar M, Sun Q, Yang D, Borowiak M, Sumazin P, Elsea SH, Bissig-Choisat B, Bissig KD Reprogramming metabolic pathways in vivo with CRISPR/Cas9 genome editing to treat hereditary tyrosinaemia. Nat Commun. 2016 August 7:12642. PMID: 27572891

Many metabolic liver disorders are refractory to drug therapy and require liver transplantation. Here we demonstrated a new strategy, which we call metabolic pathway reprogramming, to treat hereditary tyrosinemia type I in mice; rather than edit the disease-causing gene, we delete a gene in a disease-associated pathway to render the phenotype benign. Using CRISPR/Cas9 in vivo, we converted hepatocytes from having the neonatally fatal tyrosinemia type I to having the benign tyrosinemia type III by deleting Hpd (hydroxyphenylpyruvate dioxigenase). Hpd excision successfully reroutes tyrosine catabolism, leaving treated mice healthy and asymptomatic. Metabolic pathway reprogramming sidesteps potential difficulties associated with editing a critical disease-causing gene and can be explored as an option for treating other diseases

Bissig-Choisat B, Kettlun-Leyton C, Legras XL, Zorman B, Barzi M, Chen LL, Amin MD, Huang YH, Pautler RG, Hampton OA, Prakash MM, Muzny D, Doddapaneni H, Hu J, Shi Y, Gaber WM, Hicks MJ, Thompson PA, Lu Y, Mills GB, Finegold M, Goss JA, Parsons DW, Vasudevan SA, Sumazin P, Lopez-Terrada D, Bissig KD Metastatic patient-derived xenograft model and isogenic cell lines for pediatric liver cancer research. J Hepatol. 2016 April 65(2):325-33. PMID: 27117591

Pediatric liver cancer is a rare but serious disease that is poorly understood; no experimental animal model has been able to replicate the behavior and molecular mechanisms driving these tumors. In this paper, we developed a new orthotopic technique that enabled us to graft a series of patient-derived tumor cells into mice, from all major subtypes of pediatric liver cancer: hepatoblastoma, hepatocellular cancer, and hepatocellular malignant neoplasm. We also developed stem cell culturing techniques to derive new pediatric cancer cell lines from the xenografted mice. This study demonstrated that the patient-derived tumor xenografts recapitulate the histological, genetic, biological, and behavioral characteristics of the primary tumors. We tested a therapy for an aggressive hepatocellular malignant neoplasm with the MEK1 inhibitor trametinib and the IPI3 kinase inhibitor NVP-BKM120, which strongly inhibited tumor growth. This model is thus a valuable tool for studying tumor biology and patient-specific therapeutic responses.

Barzi M, Pankowicz FP, Zorman B, Liu X, Legras X, Yang D, Borowiak M, Bissig-Choisat B, Sumazin P, Li F, Bissig KD. A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism." Nat Commun. 2017 June 28;39:s41467-017. PMID: 28659616

Only one out of 10 drugs in development passes clinical trials. Many fail because experimental animal models do not replicate human drug metabolism. Human liver chimeric mice are a step forward in this regard, as the human hepatocytes in chimeric livers generate human metabolites, but the remaining murine hepatocytes contain an expanded set of P450 cytochromes that form the major class of drug-metabolizing enzymes. We therefore generated a conditional knock-out of the NADPH-P450 oxidoreductase (Por) gene in our humanized (PIRF) mice. Here we show that homozygous PIRF mouse livers are readily repopulated with human hepatocytes, and when the murine Por gene is deleted, they predominantly use human cytochrome metabolism. When given the anticancer drug gefitinib or the retroviral drug atazanavir, the Por-deleted humanized PIRF mice develop higher levels of the major human metabolites than current models. Humanized, murine Por-deficient PIRF mice can thus predict human drug metabolism and should be useful for preclinical drug development.

Pankowicz FP, Barzi M, Kim KH, Legras X, Martins CS, Wooton-Kee CR, Lagor WR, Marini JC, Elsea SH, Bissig-Choisat B, Moore DD, Bissig KD. Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing Gastroenterology. 2018 June 155(6):1967-1970. Pubmed PMID: 30170115

Despite tremendous advances in gene editing, it remains time-consuming and technically challenging to knock out genes in specific tissues. Here we developed an approach that enables any lab to use a CRISPR/Cas9 system to disrupt genes in livers of adult mice within just a few months. We used this somatic liver knockout (SLiK) approach to create a liver-specific knockout of argininosuccinate lyase; the mice develop hyperammonemia, just as do human patients with the same defect. We also used the SLiK system to knock out expression of five different enzymes involved in drug metabolism within the same mouse liver —something that would be exceedingly difficult to do with traditional mouse transgenic tools.

Journal articles

Valanejad L, Cast A1, Wright M, Bissig KD, Karns R, Weirauch MT, Timchenko N. PARP1 activation increases expression of modified tumor suppressors and pathways underlying development of aggressive hepatoblastoma. Commun Biol. 2018 June 1:67. Pubmed PMID: 30271949

Kho J, Tian X, Wong WT, Bertin T, Jiang MM, Chen S, Jin Z, Shchelochkov OA, Burrage LC, Reddy AK, Jiang H, Abo-Zahrah R, Ma S, Zhang P, Bissig KD, Kim JJ, Devaraj S, Rodney GG, Erez A, Bryan NS, Nagamani SCS, Lee BH. Argininosuccinate Lyase Deficiency Causes an Endothelial-Dependent Form of Hypertension AJHG. 2018 August 103(2):276-287. Pubmed PMID: 30075114

Kruse RL, Shum T, Tashiro H, Barzi M4, Yi Z, Whitten-Bauer C, Legras X, Bissig-Choisat B, Garaigorta U, Gottschalk S, Bissig KD. HBsAg-redirected T cells exhibit antiviral activity in HBV-infected human liver chimeric mice. Cytotherapy. 2018 May 20(5):697-705. Pubmed PMID: 29631939

Woodfield SE, Shi Y, Patel RH, Jin J, Major A, Sarabia SF, Starosolski Z, Zorman B, Gupta SS, Chen Z, Ibarra AM, Bissig KD, Ghaghada KB, Sumazin P, López-Terrada D, Vasudevan SA. A Novel Cell Line Based Orthotopic Xenograft Mouse Model That Recapitulates Human Hepatoblastoma. Sci Rep. 2017 December 7(1):1775. Pubmed PMID: 29259231

Kruse RL, Shum T, Legras X, Barzi M, Pankowicz FP, Gottschalk S, Bissig KD. In Situ Liver Expression of HBsAg/CD3-Bispecific Antibodies for HBV Immunotherapy. Mol Ther Methods Clin Dev. 2017 August 31;7:32-41. Pubmed PMID: 29018834

Velasquez-Mao AJ, Tsao CJM, Monroe MN, Legras X, Bissig-Choisat B, Bissig KD, Ruano R, Jacot JG. "Differentiation of spontaneously contracting cardiomyocytes from non-virally reprogrammed human amniotic fluid stem cells." PLoS One. 2017 May 17;12(5):e0177824. Pubmed PMID: 28545044

Pankowicz FP, Jarrett KE, Lagor WR, Bissig KD. "CRISPR/Cas9: at the cutting edge of hepatology." Gut. 2017 July;66(7):1329-1340. Pubmed PMID: 28487442

Reviews, commentaries, and invited chapters

Akkina R, Barber DL, Bility MT, Bissig KD, Burwitz BJ, Eichelberg K, Endsley JJ, Garcia JV, Hafner R, Karakousis PC, Korba BE, Koshy R, Lambros C, Menne S, Nuermberger EL, Ploss A, Podell BK, Poluektova LY, Sanders-Beer BE, Subbian S, Wahl A. Small Animal Models for Human Immunodeficiency Virus (HIV), Hepatitis B, and Tuberculosis: Proceedings of an NIAID Workshop. Pubmed PMID: 10.2174/1570162X18666191223114019